Western blot analysis of TMEM119 in extracts of various cell lines. Samples were incubated with TMEM119 Polyclonal antibody (Product # PA5-120705) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Western Blot analysis of TMEM119 in various cell lines using a TMEM119 Polyclonal antibody (Product # PA5-120705) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.
Supplier Page from Thermo Fisher Scientific for TMEM119 Antibody